Caracterização epidemiológica de acidentes com animais peçonhentos entre 2012-2021: revisão sistemática / Epidemiological characterization of accidents with poisonous animals between 2012-2021: systematic review / Caracterización Epidemiológica de acidentes con animales venenosos entre 2012-2021: revisión sistemática

Acidentes por animais peçonhentos são considerados um problema cosmopolita de saúde pública.Poucos são os estudos de revisão acerca da temática encontrados na literatura. Objetivo:definir os principaisaspectos epidemiológicos envolvidos nos acidentes com animais peçonhentos no período 2012-2021.Metodologia:Revisão sistemática, com base no protocolo PRISMA, incluindoartigos publicados entre 2012 e 2021, nos idiomas inglês, espanhol e português, a partir da busca nas bases de dados PUBMED, SCIELO, LILACS e Portal de Periódicos CAPES, utilizando a combinação dos descritores "Venoms" AND "Accidents" AND/OR "Epidemiological".Resultados:Foram encontrados 729 artigos, dos quais 17 foram selecionados para comporem esta revisão. A maioria(64,7%)dos artigos utilizaram o Sistema de Informação de Agravos de Notificação como fonte de dados, 76,4% evidenciaram o sexo masculino como o principal acometido, 64,7% apontaram a zona rural como principal área de ocorrência e faixa etária de 21-30 anos representou 47,05% dos artigos. Acidentes ofídicos demonstraram maior prevalência(47,05%), seguidos pelos escorpiônicos(23,5%). Os membros inferiores foram os mais acometidos(76,4%). Destacou-se tempo de atendimento de uma até 6 hora. Prevaleceram acidentes leves(82,3%), com bons índices de cura e poucos óbitos quanto comparado com o número total de casos. A soroterapia foi trabalhada em 11(64,7%)estudos, sendo utilizada principalmente em acidentes com serpentes. Dor e edema foram as principais manifestações locais, alterações vagais foram as principais manifestações sistêmicas e necrose, alterações do sistema circulatório e renal foram as principais complicações apontadas.Conclusões:Os achados possibilitaram melhor visualização e entendimento da problemática, reforçando a importância do desenvolvimento de ações interventivas que venham a melhorar os sistemas de saúde locais, colaborando na identificação de falhas e melhorias no atendimento imediato e preventivo em saúde (AU).

manifestations, vagal changes the main systemic manifestations, and necrosis, circulatory and renal system alterations the most important complications reported.Conclusions: The findings allow better visualization and understanding of the problem, reinforcing the importance of intervention to improve local health systems, thereby helping identify the shortcomings and improvements in prompt and preventive treatment (AU).

Los accidentes con animales ponzoñosos constituyen un problema de salud pública cosmopolita. Pocos estudios han realizado una revisión de la literatura sobre el tema.Objetivo: Determinar los principales aspectos epidemiológicos involucrados en los accidentes con animales ponzoñosos entre 2012 y 2021. Metodología: Se trata de una revisión sistemática, basada en el protocolo PRISMA, que incluye artículos publicados entre 2012 y 2021, en inglés, español y portugués, mediante búsquedas en las bases de datos PUBMED , SCIELO, LILACS y CAPES, y una combinación de los descriptores "Venenos" Y "Accidentes" Y/O "Epidemiológico". Resultados: Se encontraron un total de 729 artículos, de los cuales 17 fueron seleccionados para la revisión. La mayoría (64,7%) de los artículos utilizaron el Sistema de Información de Enfermedades de Declaración Obligatoria como fuente de datos, el 76,4% encontró que el sexo masculino fue el más afectado, el 64,7% se presentó en la zona rural y el grupo etario de 21 a 30 años representó el 47,05% de las publicaciones. Las mordeduras de serpientes fueron las más prevalentes (47,05%), seguidas de las picaduras de escorpión (23,5%), y los miembros inferiores fueron los más afectados (76,4%). El tiempo de tratamiento fue de hasta 6 horas. Las mordeduras leves fueron las más comunes (82,3%), con buenas tasas de curación y pocas muertes en comparación con el número de casos. La seroterapia se aplicó en 11 (64,7%) estudios, principalmente en mordeduras de serpientes. El dolor y la tumefacción fueron las principales manifestaciones locales, los cambios vágales las principales manifestaciones sistémicas y la necrosis, las alteraciones del sistema circulatorio y renal las complicaciones más importantes reportadas.Conclusiones: Los resultados permiten una mejor visualización y comprensión del problema, reforzando la importancia de la intervención para mejorar los sistemas locales de salud, ayudando así a identificar las falencias y mejoras en el tratamiento oportuno y preventivo (AU).

Trends in occurrences of accidents by venomous animals in brazil: analysis of notifications from 2007 to 2021 / Tendências na ocorrência de acidentes por animais peçonhentos no brasil: análise das notificações de 2007 a 2021 / Tendencias en las ocurrencias de accidentes por animales venenosos en Brasil: análisis de las notificaciones de 2007 a 2021

Os acidentes por animais peçonhentos sãoum problema de saúde pública que apresenta elevado número de casos anuais no Brasil. Objetivo:descrever e analisar as ocorrências de acidentes por animais peçonhentos no Brasil e suas tendências entre 2007 e 2021.Metodologia:oestudo utilizou dados doSistema de Informação de Agravos de Notificação edo Sistema de Informação sobre Mortalidade.A população do estudo foi analisada segundo sexo, faixa etária e região de residência. A análise de tendência da série histórica foi realizada por regressão linear generalizada usando a estimação de Prais-Winsten.Resultados:as taxas de ocorrência padronizadas dobraram no período estudado (53,8/100.000 hab. em 2007 para 111,2/100.000 hab. em 2021). A região Nordeste apresentou a maior taxa de ocorrência padronizada (105,5/100.000 hab.). As regiões Nordeste, Sudeste e Centro-Oeste apresentaram maior taxa bruta de acidentes por escorpiões (75,8, 48,7 e 32,8 acidentes/100.000 hab., respectivamente), enquanto a região Norte por serpentes (54,2 acidentes/100.000 hab.) e a Região Sul por aranhas (60,3 acidentes/100.000 hab.). A taxa de mortalidade observada no período de 2007 a 2020 foi de 1,3 óbitos/1 milhão de hab. Entre as mortes registradas, 39,6% foram por picada de cobra.Conclusões:as tendências no Brasil foram ascendentes para ambos os sexos e para todas as faixas etárias. Apenas as regiões Norte e Sul apresentaram algumas categorias com tendências estacionárias. A análise dos dados epidemiológicos possibilita a identificação de populações-alvo e a elaboração de políticas públicas a fim de prevenir novos acidentes, bem como aprimorar o atendimento às pessoas acometidas por meio do planejamento da distribuição de soros antiofídicos (AU).

Accidents by venomous animals is a public health problem that presents a high number of annual cases in Brazil.Objective:to describe and analyze the occurrences of accidents by venomous animalsin Brazil and their trends between 2007 and 2021.Methodology:the study used Notifiable Diseases Information Systemand Mortality Information Systemdata. The study population wasanalyzed according to sex, age group, and region of residence. Trend analysis of the historical series was performed by generalized linear regression using Prais-Winsten estimation.Results:the standardized occurrence rates doubled during the studied period (53.8/100,000 inhab. in 2007 to 111.2/100,000 inhab. in 2021). The Northeast region had the highest standardized occurrence rate (105.5/100,000 inhab.). The Northeast, Southeast, and Midwest regions had a higher crude rate of accidents caused by scorpions (75.8, 48.7 and 32.8 accidents/100,000 inhab., respectively), while the North region by snakes (54.2 accidents/100,000 inhab.), and the South region by spiders (60.3 accidents/100,000 inhab.). Death rate observed in the period from 2007 to 2020 was 1.3deaths/1 million inhab. Among the deaths registered, 39.6% were from snakebite.Conclusions:trends in Brazil were ascendant for both sexes and for all age groups. Only the North and South regions showed some categories with stationary trends. The analysis of epidemiological data makes it possible the identification of target populations and elaborations of public policies in order to prevent new accidents, as well as the improvement of care for affected people by planning the distribution of antivenom serums (AU).

Los accidentes por animales venenosos son un problema de salud pública que presenta un elevado número de casos anuales en Brasil. Objetivo: describir y analizar lasocurrencias de accidentes por animales venenosos en Brasil y sus tendencias entre 2007 y 2021.Metodología: el estudio utilizó datos del sistema de información de enfermedades de declaración obligatoria(SINAN, según su sigla en portugués)ydelsistema de información de mortalidad. La población delestudio se analizó según sexo, grupo de edad y región de residencia. El análisis de tendencia de la serie histórica se realizó mediante regresión lineal generalizada utilizando la estimación de Prais-Winsten.Resultados: las tasas de ocurrencia estandarizadas se duplicaron durante el período estudiado (de 53,8/100000 hab. en 2007 a 111,2/100000 hab. en 2021). La región Nordeste presentó la mayor tasa de ocurrencia estandarizada (105,5/100000 hab.). Las regiones Nordeste, Sudeste y Medio Oeste presentaron la mayor tasa bruta de accidentes por alacranes (75,8, 48,7 y 32,8 accidentes/100000 hab., respectivamente), mientras que la región Norte por serpientes (54,2 accidentes/100000 hab.), y la Región Sur por arañas (60,3 accidentes/100000 hab.). La tasa de mortalidad observada en el período de 2007 a 2020 fue de 1,3 muertes/1 millón de hab. Entre las muertes registradas, el 39,6% fueron por mordedura de serpiente.Conclusiones: las tendencias en Brasil fueron ascendentes para ambos sexos y para todos los grupos de edad. Solo las regiones Norte y Sur presentaron algunas categorías con tendencias estacionarias. El análisis de datos epidemiológicos posibilita la identificación de poblaciones objetivo y la elaboración de políticas públicas para la prevención de nuevos accidentes, así como la mejora de la atención a las personas afectadas mediante la planificación de la distribución de sueros antiofídicos (AU).

Actitudes hacia el aborto deseado en estudiantes de ciencias de la salud chilenos / Attitudes towards wanted abortion among Chilean health sciences students

Introducción: La interrupción del embarazo mediando principalmente la voluntad de la mujer, es decir, cuando una mujer desea abortar para terminar su embarazo por cualquier razón, es un procedimiento controversial. Las actitudes individuales de los profesionales de la salud hacia este procedimiento influirían sobre la aceptación o el rechazo de realizarlo y, por ende, afectarían su acceso en el sistema de salud. Objetivo: Relacionar actitudes hacia la interrupción del embarazo con características sociodemográficas y académicas de estudiantes chilenos de enfermería, medicina y obstetricia y puericultura. Método: Estudio cuantitativo con diseño observacional, transversal y descriptivo. Reclutamos estudiantes universitarios con un muestreo no probabilístico. Recolectamos datos mediante cuestionarios virtuales autoadministrados. Preguntamos por la intención de realizar o asistir un aborto médico o quirúrgico frente a 15 escenarios distintos y creamos un índice con estas respuestas. Calculamos estadísticas descriptivas básicas y creamos modelos de regresión lineal. Consideramos significancia estadística si p < 0,05. Resultados: Participaron 229, 306 y 233 estudiantes de enfermería, medicina y obstetricia y puericultura, respectivamente (en total, 768). En el modelo de regresión lineal múltiple, declararse cristiano (β = −0,248) y afirmar que la religión es muy o totalmente importante en la vida (β = −0,269) se asociaron más fuertemente y de manera inversa y significativa con el índice de intención de realizar o asistir un aborto médico o quirúrgico. Conclusiones: La religión es un factor que influiría decisivamente sobre las actitudes hacia la interrupción del embarazo. Los escenarios más positivamente valorados podrían explicarse considerando que las leyes reflejarían los valores predominantes de una sociedad.

Introduction: Termination of pregnancy mediated primarily by the womans will, i.e., when a woman wishes to have an abortion to terminate her pregnancy for any reason, is a controversial procedure. The individual attitudes of health professionals towards this procedure would affect the degree of acceptance or rejection of performing this procedure and, therefore, would affect its accessibility in the health system. Objective: To relate attitudes towards abortion with sociodemographic and academic characteristics of Chilean nursing, medicine and midwifery students. Method: Quantitative study with observational, cross-sectional and descriptive design. We recruited university students with non-probabilistic sampling. We collected data through self-administered virtual questionnaires. We asked about the intention to perform an abortion in 15 different scenarios and created an index with these responses. We calculated basic descriptive statistics and created linear regression models. We considered statistical significance if p < 0.05. Results: 229, 306 and 233 students from nursing, medicine and midwifery participated, respectively (total: 768). In the multiple linear regression model, declaring oneself a Christian (β = −0.248) and stating that religion is very or totally important in life (β = −0.269) were inversely and significantly associated with the index of intention to perform an abortion. Conclusions: Religion is a factor that would decisively influence attitudes toward termination of pregnancy. The more positively valued scenarios could be explained by considering that laws would reflect the predominant values of a society.

Vértigo en gestantes: presentación clínica, impacto en la calidad de vida y factores asociados / Vertigo in pregnancy: clinical presentation, impact on quality of life and associated factors

OBJETIVO: Evaluar las características clínicas, el impacto en la calidad de vida y los factores asociados con vértigo en gestantes hospitalizadas en una institución de alta complejidad. MÉTODO: Estudio transversal. Se realizó una encuesta dirigida a la presencia de vértigo y sus características clínicas en 2020-2021. La calidad de vida se evaluó con el cuestionario Dizziness Handicap Inventory (DHI). El análisis estadístico incluyó un modelo lineal generalizado. RESULTADOS: De 103 mujeres, el 19,4% indicaron vértigo principalmente en el segundo trimestre de gestación (60%), con una mediana de 3,5 (rango intercuartil: 1,5-7,5) episodios. Fueron referidos vértigos episódicos asociados con cambios posicionales (40%), acompañados de inestabilidad (60%), cefalea (60%), fotopsias (55%) y tinnitus (45%). Las mujeres con vértigo presentaron mayor frecuencia de discapacidad moderada a grave en las dimensiones del DHI emocional (30 vs. 2,4%; p = 0,001), funcional (40 vs. 2,4%; p < 0,001) y física (55 vs. 2,4%; p < 0,001) en comparación con las mujeres sin la patología. La hospitalización durante el embarazo Razón de proporción (RP): 4,02; intervalo de confianza del 95% [IC95%]: 1,64-9,85; p = 0,002) y la presencia de vértigo pregestacional (RP: 2,37; IC95%: 1,15-4,88; p = 0,019) se identificaron como factores asociados. CONCLUSIONES: La alta frecuencia de vértigo en las gestantes sugiere la importancia de estudiar esta condición durante el embarazo, para lograr un manejo integral y generar acciones de prevención y control efectivas.

OBJECTIVE: To evaluate clinical characteristics, impact on quality of life and factors associated with vertigo in pregnant women hospitalized in a highly complex institution. METHOD: A cross-sectional study was conducted in 2021-2022. One focused survey including Dizziness Handicap Inventory (DHI) was performed. The statistical analysis was performed using a generalized lineal regression. RESULTS: 103 patients were included, 19.4% indicated vertigo mostly during the second semester (60%). A median of 3.5 episodes was obtained (RIC: 1.5-7.5). Positional and episodic vertigos (40%) associated with unsteadiness (60%), headache (60%), photopsia (55%) and tinnitus (45%) were described. DHI in pregnant females with vertigo compared to those without vertigo, presented higher rates of moderate to severe disability in the emotional (30 vs. 2.4%: p = 0.001), functional (40 vs. 2.4%; p < 0.001) and physical (55 vs. 2.4%; p < 0.001) dimensions. Hospitalizations during the pregnancy (RP: 4.02; 95%CI: 1.64-9.85; p = 0.002) and previous episodes before pregnancy (RP: 2.37; 95%CI: 1.15-4.88; p = 0.019) were identified as associated factors with current vertigo episodes. CONCLUSIONS: The high frequency of vertigo in pregnant women suggests the importance of studying this condition during pregnancy, to achieve comprehensive management and generate effective prevention and control actions.

Nationwide Stature Estimation from Forearm Length Measurements in Montenegrin Adolescents / Estimación de la Estatura a Partir de Mediciones de la Longitud del Antebrazo en Adolescentes Montenegrinos

SUMMARY: The purpose of this research is to determine a regression equation for estimation of stature from forearm length measurements. This research was carried out on 1200 subjects (604 male and 596 female) among the population of Montenegrin adolescents. The stature and forearm length measurements were taken according to the ISAK protocol, and the data were analyzed statistically. Linear regression analysis determined the prediction of forearm length on the criterion variable a body height at the significance level of p <0.05. These relations are presented in the form of scatter diagram. Thereby, we obtained the coefficient of determination, the multiple correlation coefficients, the partial correlation coefficient, the regression, t-test and standardized beta coefficient. The results of this research study confirmed that forearm length reliably predicts stature in both sexes of Montenegrin adolescents and revealed a very useful finding for physical anthropologists and experts from related fields. It was confirmed that there is a correlation between forearm length and body height (males: 31.9 %, females: 33.3 %).

El propósito de esta investigación fue determinar una ecuación de regresión para la estimación de la estatura a partir de medidas de la longitud del antebrazo. Esta investigación se llevó a cabo en 1200 sujetos (604 hombres y 596 mujeres) entre la población de adolescentes montenegrinos. Las medidas de estatura y longitud del antebrazo se tomaron de acuerdo con el protocolo ISAK y los datos se analizaron estadísticamente. El análisis de regresión lineal determinó la predicción de la longitud del antebrazo en la variable de criterio una altura del cuerpo en el nivel de significación de p <0,05. Estas relaciones se presentan en forma de diagrama de dispersión. De tal manera obtuvimos el coeficiente de determinación, los coeficientes de correlación múltiple, el coeficiente de correlación parcial, la regresión, la prueba t y el coeficiente beta estandarizado. Los resultados de este estudio confirmaron que la longitud del antebrazo predice de manera confiable la estatura en adolescentes montenegrinos de ambos sexos y reveló un hallazgo muy útil para los antropólogos físicos y expertos en áreas relacionadas. Se confirmó que existe una correlación entre la longitud del antebrazo y la altura del cuerpo (hombres: 31,9 %, mujeres: 33,3 %).

Sistema de cotas e desempenho acadêmico: estudo seccional em uma universidade pública brasileira / Quota system and academic performance: sectional study in a brazilian public university / Sistema de cuotas y rendimiento académico: estudio seccional en una universidad pública brasileña

Introdução: A lei de cotas (nº 12.711/2012) foi criada com o intuito de oportunizar o acesso de alunos oriundos de escolas públicas ao ensino superior. Desde então, medidas adicionais foram tomadas para garantir, não só o acesso, mas a permanência destes alunos nas universidades. Objetivo: Este estudo teve como objetivo avaliar o desempenho acadêmico dealunos cotistas e não cotistas da Faculdade de Odontologia da Universidade Federal do Pará. Metodologia: Foram coletados dados de alunos matriculados nos anos de 2020 e2021 por meio de um questionário on-line e realizada a análise de histórico acadêmico dos voluntários. A análise dos dados foi realizada com software Jamovi versão 1.6.23, utilizando os testesQui-quadrado, t de Student para amostras independentes e análise de regressão linear multivariada. Em todas as análises foi adotado o nível de significância de 0,05. Resultados: Com uma taxa de resposta de 39,92% (n=200), os resultados demonstraram haver um melhor desempenho acadêmico para o grupo não-cotista no que se refere ao Coeficiente de Rendimento Geral (p=0,001). Além disso, alunos do grupo cotista enfrentam mais dificuldades quando comparados aos não-cotistas (p<0,0001). Na análise múltipla verificou-se que juntas, forma de ingresso (cotista ou não), o enfrentamento de dificuldades, recebimento de auxílios e atividades fora da Faculdade explicam quase 10% da variável Coeficiente de Rendimento Geral (R² = 0,098). Conclusões: A complexidade da comparação sugere a possibilidade de múltiplas causas, entre elas o fator socioeconômico e outras dificuldades experienciadas. Entender e atuar nestas causas é de fundamental importância para a construção de uma universidade inclusiva de qualidade (AU).

Introduction:The quota law (nº 12.711/2012) was created with the objective of providing opportunities for students from public schools to enter federal universities. Since then, additional measures have been taken to ensure not only the access, but the permanence of these students in universities. Objectives:This study aimed to assess the academic performance of quota students and non-quota students at the Faculty of Dentistry of the Federal University of Pará. Methods:Data were collected from students enrolled in the years 2020 and 2021 through an online questionnaire and the academic history of the volunteers was analyzed. Data analysis was performed with Jamovi software version 1.6.23, using the chi-square test, Student's t test for independent samples, and multivariate linear regression analysis. In all analyses, a significance level of 0.05 was adopted. Results:With a response rate of 39.92% (n=200), the results showed a better academic performance for the non-quotastudents with regard to the General PerformanceCoefficient (p=0.001). In addition, quota students face more difficulties when compared to non-quota students (p<0.0001). In the multiple analysis, it was found that the admission modality (quota students or non-quota students), facing difficulties, receiving aid, and activities outside the University explained together almost 10% of the General Performance Coefficient variable (R² = 0.098). Conclusion:The complexity of the comparison suggests the possibility of multiple causes, including the socioeconomic factor and other difficulties experienced. Understanding and acting on these causes is of fundamental importance for the construction of a quality inclusive university (AU).

Introducción: La ley de cuotas (nº 12.711/2012) fue creada con el objetivo de brindar a estudiantes de colegios públicosla oportunidad de ingresar a una universidad federal. Desde entonces, se han tomado medidas adicionales para asegurar, no solo el acceso, sino también la permanencia de estos estudiantes en las universidades. Objetivos:Este estudio tuvo como objetivo evaluar el rendimiento académico de los alumnos con cuota y los sin cuota de la Facultad de Odontología de la Universidad Federal de Pará. Métodos:Se recolectaron datos de estudiantes matriculados en los años 2020 y 2021 a través de un cuestionario en línea y se realizó el análisis del historial académico de los voluntarios. El análisis de datos se realizó con el software Jamovi versión 1.6.23, utilizando la prueba de qui-cuadrado, prueba t de Student para muestras independientes y análisis de regresión lineal multivariado. En todos los análisis se adoptó un nivel de significación de 0.05. Resultados:Con una tasa de respuesta del 39.92% (n=200), los resultados mostraron un mejor rendimiento académico de los alumnos sin cuotacon respecto al Coeficiente de Rendimiento General (p=0.001). Además, los estudiantes con cuota enfrentan más dificultades cuando comparados a los estudiantes sin cuota (p<0.0001). En el análisis múltiple, se encontró que,en conjunto, la forma de ingreso (alumnos con cuota o sin cuota), enfrentar las dificultades, recibir ayudas y actividades fuera de la Facultad explican casi el 10% de la variable Coeficiente de Rendimiento General(R² = 0,098). Conclusión:La complejidad de la comparación sugiere la posibilidad de múltiples causas, incluido el factor socioeconómico y otras dificultades experimentadas. Comprender y actuar sobre estas causas es de fundamental importancia para la construcción de una universidad inclusiva de calidad (AU).

Comparison of Accuracy Between Pulp/Tooth Ratio and Tooth Coronal Index Methods for Dental Age Estimation Using Digital Panoramic Radiographs

ABSTRACT Objective: To evaluate the reliability and the accuracy of Tooth Coronal Index (TCI) and Pulp/Tooth Ratio (PTR) methods in dental age estimation using digital panoramic radiography. Material and Methods: In this cross-sectional study, 237 dental panoramic images were collected. The two methods (TCI and PTR) were applied to all left mandibular first and second molars based on inclusion and exclusion criteria. In order to analyze the acquired data, statistical methods were used. The estimated ages derived by exclusive formula were compared to the chronological age, and the error ranges for each indicator were measured to determine their accuracy. Results: There were negative correlations between PTR in the first molar (r=-0.89) and in the second molar (r=-0.788), as well as TCI in the first molar (r=-0.587) and in the second molar (r=-.242). In this study, we found that the Pulp/Tooth Ratio (PTR) accuracy rate for mandibular first and second molar teeth was 79.21% and 62.09 %, respectively, although the Tooth Coronal Index (TCI) value for these teeth was 34.45% and 5.85%. Conclusion: Pulp/Tooth Ratio and Tooth Coronal Index are potential age estimation indices. Although PTR was the more accurate one in our study. The results also demonstrated that indices related to the first molar tooth could be used to estimate age with greater accuracy and validity.

A novel differential diagnosis algorithm for chronic lymphocytic leukemia using immunophenotyping with flow cytometry

Abstract Introduction The availability of a clinical decision algorithm for diagnosis of chronic lymphocytic leukemia (CLL) may greatly contribute to the diagnosis of CLL, particularly in cases with ambiguous immunophenotypes. Herein we propose a novel differential diagnosis algorithm for the CLL diagnosis using immunophenotyping with flow cytometry. Methods The hierarchical logistic regression model (Backward LR) was used to build a predictive algorithm for the diagnosis of CLL, differentiated from other lymphoproliferative disorders (LPDs). Results A total of 302 patients, of whom 220 (72.8%) had CLL and 82 (27.2%), B-cell lymphoproliferative disorders other than CLL, were included in the study. The Backward LR model comprised the variables CD5, CD43, CD81, ROR1, CD23, CD79b, FMC7, sIg and CD200 in the model development process. The weak expression of CD81 and increased intensity of expression in markers CD5, CD23 and CD200 increased the probability of CLL diagnosis, (p < 0.05). The odd ratio for CD5, C23, CD200 and CD81 was 1.088 (1.050 - 1.126), 1.044 (1.012 - 1.077), 1.039 (1.007 - 1.072) and 0.946 (0.921 - 0.970) [95% C.I.], respectively. Our model provided a novel diagnostic algorithm with 95.27% of sensitivity and 91.46% of specificity. The model prediction for 97.3% (214) of 220 patients diagnosed with CLL, was CLL and for 91.5% (75) of 82 patients diagnosed with an LPD other than CLL, was others. The cases were correctly classified as CLL and others with a 95.7% correctness rate. Conclusions Our model highlighting 4 markers (CD81, CD5, CD23 and CD200) provided high sensitivity and specificity in the CLL diagnosis and in distinguishing of CLL among other LPDs.

Knowledge and practices surrounding outbreaks and COVID-19 among community health workers in rural Rwanda: a cross-sectional mixed-methods study

Introduction: Community Health Workers (CHW) are a critical resource for outbreak preparedness and response. However, CHWs´ ability to respond to outbreaks depends on their accurate knowledge of the disease and proper adoption of disease prevention practices. We explored knowledge and practices related to outbreaks in general, and COVID-19 among CHWs in Rwanda. Methods: this cross-sectional multimethod study used stratified simple random sampling to recruit three cadres of CHWs (agents de santé maternelle, female Binomes, and male Binomes ) from three rural Rwandan districts. We used telephone-based data collection to administer quantitative surveys (N=292) and qualitative interviews (N=24) in September 2020. We calculated descriptive statistics and conducted thematic analysis of qualitative data. We assessed for associations between general outbreak-related knowledge and receipt of training using Chi-square tests and between COVID-19 related knowledge and CHW characteristics and adoption of prevention methods using linear regression models. Results: only 56.2% of CHWs had received training on any health topic in 12 months prior to COVID-19 pandemic and only 19.2% had specifically received training on outbreak preparedness. Almost all CHWs reported preventing COVID-19 by wearing facemasks (98%), washing hands (95%), and social distancing in crowds (89%) with fewer reporting staying at home (50%), sneezing or coughing into an elbow (38%) or using hand sanitizer (18%). Almost all CHWs in our study knew that COVID-19 transmit through respiratory droplets (98%) and by infected surfaces (98%) and that asymptomatic spread is possible (91%). However, fewer than half of community health workers correctly affirmed that children were at low risk of becoming severely ill (48%) and only 32% correctly rejected the misconception that everyone with COVID-19 would become severely ill. There was no association between COVID-19-related knowledge and adoption of COVID-19 preventative practices. Qualitative findings suggested that while CHWs possessed lots of correct information about COVID-19 and reported good adherence to COVID-19 prevention practices, they also commonly held misconceptions that over-exaggerated the dangers of COVID-19. Conclusion: gaps in knowledge, training, and access to information point to a need for additional investment in supervision and credible informational systems to support CHWs.

Recent changes in trends of mortality from cervical cancer in Southeastern Brazil / Mudanças recentes nas tendências da mortalidade por câncer de colo do útero no Sudeste do Brasil

ABSTRACT OBJECTIVE To analyze the trends of cervical cancer mortality in Brazilian Southeastern states, and to compare them to Brazil and other regions between 1980 and 2020. METHODS Time series study based on data from the Sistema de Informações de Mortalidade (Brazilian Mortality Information System). Death data were corrected by proportional redistribution of deaths from ill-defined causes and cervical cancer of unspecified portion. Age-standardized and age-specific rates were calculated by screening target (25-39 years; 40-64 years) and non-target (65 years or older) age groups. Annual percentage changes (APC) were estimated by linear regression model with breakpoints. The coverage of Pap Smear exam in the Unified Health System (SUS) was evaluated between 2009 and 2020 according to age group and locality. RESULTS There were increases in corrected mortality rates both in 1980 and in 2020 in all regions, with most evident increments at the beginning of the series. There was a decrease in mortality nationwide between 1980-2020; however, the state of São Paulo showed a discrete upward trend in 2014-2020 (APC=1.237; 95%CI 0.046-2.443). Noteworthy is the trend increment in the 25-39 year-old group in all study localities, being sharper in the Southeast region in 2013-2020 (APC=5.072; 95%CI 3.971-6.185). Screening coverage rates were highest in São Paulo and lowest in Rio de Janeiro, with a consistent decline from 2012 onwards at all ages. CONCLUSIONS São Paulo is the first Brazilian state to show a reversal trend in mortality from cervical cancer. The changes in mortality patterns identified in this study point to the need for reorganization of the current screening program, which should be improved to ensure high coverage, quality, and adequate follow-up of all women with altered test results.

RESUMO OBJETIVO Analisar as tendências da mortalidade por câncer de colo de útero nos estados da região Sudeste e compará-las com o Brasil e demais regiões entre 1980 e 2020. MÉTODOS Estudo de série temporal com base nos dados do Sistema de Informações de Mortalidade. Os dados de óbito foram corrigidos por redistribuição proporcional das mortes por causas mal definidas e por câncer de útero de porção não especificada. Foram calculadas taxas padronizadas por idade e específicas por faixas etárias alvo de rastreamento (25-39 anos; 40-64 anos) e não alvo (65 anos ou mais). Variações percentuais anuais foram estimadas por modelo de regressão linear com pontos de quebra. A cobertura do exame Papanicolaou no Sistema Único de Saúde (SUS) foi avaliada entre 2009 e 2020 segundo faixa etária e localidade. RESULTADOS Foram verificados aumentos das taxas de mortalidade corrigidas tanto em 1980 como em 2020 em todas as regiões, com incrementos mais evidentes no início da série. Houve queda da mortalidade em todo o país entre 1980-2020, entretanto, o estado de São Paulo apresentou discreta tendência de aumento em 2014-2020 (APC=1,237; IC95% 0,046-2,443). Destaca-se o incremento da tendência no grupo de 25-39 anos em todas as localidades de estudo, mostrando-se mais acentuado na região Sudeste em 2013-2020 (APC=5,072; IC95% 3,971-6,185). As taxas de cobertura de rastreamento foram mais elevadas em São Paulo e mais baixas no Rio de Janeiro, com queda consistente a partir de 2012 em todas as idades. CONCLUSÕES São Paulo é o primeiro estado brasileiro a apresentar inversão de tendência da mortalidade por câncer de colo do útero. As mudanças nos padrões de mortalidade identificadas neste estudo apontam para a necessidade de reorganização do atual programa de rastreamento, que deve ser aperfeiçoado para garantir alta cobertura, qualidade e seguimento adequado de todas as mulheres com exames alterados.

Global burden and trends of lung cancer incidence and mortality / 中华医学杂志(英文版)

BACKGROUND@#Lung cancer has been the leading cause of cancer-related deaths worldwide for many years. This study aimed to investigate the global patterns and trends of lung cancer.@*METHODS@#Lung cancer incidence and mortality were derived from the GLOBOCAN 2020 database. Continuous data from Cancer Incidence in Five Continents Time Trends were used to analyze the temporal trends from 2000 to 2012 using Joinpoint regression, and average annual percent changes were calculated. The association between the Human Development Index and lung cancer incidence and mortality was assessed by linear regression.@*RESULTS@#An estimated 2.2 million new lung cancer cases and 1.8 million lung cancer-related deaths occurred in 2020. The age-standardized incidence rate (ASIR) ranged from 36.8 per 100,000 in Demark to 5.9 per 100,000 in Mexico. The age-standardized mortality rate (ASMR) varied from 32.8 per 100,000 in Poland to 4.9 per 100,000 in Mexico. Both ASIR and ASMR were approximately twice higher in men than in women. The ASIR of lung cancer showed a downward trend in the United States of America (USA) between 2000 and 2012, and was more prominent in men. The age-specific incidence rates of lung cancer for ages of 50 to 59 years showed an upward trend in China for both men and women.@*CONCLUSIONS@#The burden of lung cancer is still unsatisfactory, especially in developing countries like China. Considering the effectiveness of tobacco control and screening in developed countries, such as the USA, there is a need to strengthen health education, accelerate the establishment of tobacco control policies and regulations, and improve early cancer screening awareness to reduce the future burden of lung cancer.

Effect of health management service for hypertension patients under framework of Basic Public Health Service Project: a regression discontinuity design / 中华流行病学杂志

Objective: To evaluate the effect of health management service on hypertension patients (HMSFHP) under the framework of the Basic Public Health Service Project by using regression discontinuity design. Methods: The participants were enrolled from an observational cohort survey in 2015 and followed up was conducted in 2019. The participants with SBP 130-150 mmHg and/or DBP 80-100 mmHg in the baseline survey of the cohort in 2015 were included in the present study. Additionally, we obtained the dates of participants receiving HMSFHP and their blood pressure data from follow-up records, physical examination records and telephone interview. The participants were divided into intervention group and control group based on the cutoff points, i.e. SBP ≥140 mmHg and/or DBP ≥90 mmHg. The local linear regression model were used to estimate the effect of HMSFHP on reducing blood pressure of the participants. Results: After adjusting for age, sex and time length of receiving HMSFHP, the results of the model including participants with 80-100 mmHg for DBP in 2015 indicated that, for the participants who received HMSFHP, the DBP decreased by 6.66 mmHg from 2015 to 2019. For the participants with SBP 130-150 mmHg in 2015, the reduction estimate of the model was -6.17 mmHg, the difference was not significant (P=0.178), suggesting that receiving HMSFHP did not cause change in SBP for the participants who received HMSFHP. Conclusion: Receiving HMSFHP had effect to reduce DBP, and HMSFHP had a positive effect on the control of blood pressure in patients with hypertension.

An examination of predictive markers for successful sperm extraction procedures: a linear model and systematic review / 亚洲男科学杂志(英文版)

The authors performed a comprehensive review of current literature to create a model comparing commonly evaluated variables in male factor infertility, for example, follicle-stimulating hormone (FSH), testicular volume (TV), and testosterone (T), to better predict sperm retrieval rate (SRR). Twenty-nine studies were included, 9 with data on conventional testicular sperm extraction (cTESE) for a total of 1227 patients and 20 studies including data on microdissection testicular sperm extraction (mTESE) for a total of 4760 patients. A weighted-means value of SRR, FSH, T, and TV was created, and a weighted linear regression was then used to describe associations among SRR, type of procedure, FSH, T, and TV. In this study, weighted-means values demonstrated mTESE to be superior to cTESE with an SRR of 51.9% vs 40.1%. Multiple weighted linear regressions were created to describe associations among SRR, procedure type, FSH, T, and TV. The models showed that for every 1.19 mIU ml-1 increase in FSH, there would be a significant decrease in SRR by 1.0%. Seeking to create a more clinically relevant model, FSH values were then divided into normal, moderate elevation, and significant elevation categories (FSH <10 mIU ml-1, 10-19 mIU ml-1, and >20 mIU ml-1, respectively). For an index patient undergoing cTESE, the retrieval rates would be 57.1%, 44.3%, and 31.2% for values normal, moderately elevated, and significantly elevated, respectively. In conclusion, in a large meta-analysis, mTESE was shown to be more successful than cTESE for sperm retrievals. FSH has an inverse relationship to SRR in retrieval techniques and can alone be predictive of cTESE SRR.

Dental Fear/Anxiety in Children and Child Emotional and Behavioural Problems

ABSTRACT Objective: To investigate the association between emotional and behavioural problems and dental fear/anxiety (DFA) in children aged four to 12 years treated at a clinic in southern Brazil. Material and Methods: In this cross-sectional study where mother-child dyads were interviewed, emotional and behavioural problems were investigated using the Strengths and Difficulties Questionnaire (SDQ) (considering five subscales). Children's DFA was evaluated through the Venham Picture Test. For each SDQ subscale, Poisson regression model was explored. Prevalence ratios (PR) were estimated, considering a significant level of p ≤ 0.05. Results: Overall, 128 children participated in this study. Most children were female (54.7%) and aged between 7 and 9 years (39.8%). The prevalence of emotional problems was 47.7% and behavioural problems were 46.1%. The prevalence of DFA was 18.8%. Children with emotional problems had a 2.3 higher prevalence of DFA (95%CI 1.06-5.04). In general, behavioural problems were not associated with DFA (95%CI 0.84-3.34) only when conduct problems were considered (2.20; 95%CI 1.02-4.70). Conclusion: Children aged between 4 and 12 years who present emotional and conduct problems tend to show higher DFA.

Psychometric properties of the short version of the Five Facets of Mindfulness Questionnaire in brazilian portuguese / Propriedades psicométricas da versão curta do Questionário das Cinco Facetas de Mindfulness em português / Propiedades psicométricas de la versión corta del Cuestionario de las Cinco Facetas de Mindfulness en portugués

The aims of the current study were to evaluate the psychometric properties of a short version of the Five Facet Mindfulness Questionnaire (FFMQ-SF-BR) and verify if the addition of wording mechanisms (positive or negative) contributes to the fit of the shortened Brazilian Portuguese version of the FFMQ. Four hundred eight participants answered self-report measurements. Structural equation modelling was employed for both objectives. Adequate model fit was found for the 5-factor FFMQ-SF-BR model, with significant improvements arising from the addition of wording mechanism effects. No evidences of differential item functioning and population heterogeneity were found. Bifactor analysis showed that latent traces are preferred overusing raw sum scores. The FFMQ-SF-BR is suitable to measure mindfulness in the Brazilian population and has the benefits of decreasing data collection length without losing content coverage. (AU)

O presente estudo objetivou avaliar as propriedades psicométricas de uma versão curta em português do Questionário das Cinco Facetas de Mindfulness (FFMQ-SF-BR) e verificar se a adição de mecanismos de formulação dos itens (positivos ou negativos) contribui para o ajuste do modelo em cinco fatores. Quatrocentos e oito participantes preencheram instrumentos de autorrelato. Modelagem de equações estruturais foi aplicada em ambos os objetivos. Índices adequados de ajuste foram encontrados para o modelo em cinco fatores, com melhorias significativas advindas da adição dos mecanismos de formulação dos itens. Não foram encontradas evidências de funcionamento diferencial dos itens ou heterogeneidade populacional. A análise de um modelo bifator demonstrou que valores de traços latentes são preferíveis à soma da pontuação. A FFMQ-SF-BR é adequada para mensurar mindfulness na população brasileira, com os benefícios de diminuir o tempo de coleta sem perda de conteúdo. (AU)

El presente estudio tuvo como objetivo evaluar las propiedades psicométricas de una versión corta del Cuestionario de las Cinco Facetas de Mindfulness (FFMQ-SF-BR) en portugués y verificar si la adición de mecanismos de formulación de ítems (positivos o negativos) contribuye al ajuste del modelo de cinco factores. Cuatrocientos ocho participantes completaron instrumentos de autoinforme. Se aplicó el modelo de ecuaciones estructurales a ambos objetivos. Se encontraron índices de ajuste adecuados para el modelo en cinco factores, con mejoras significativas derivadas de los mecanismos de formulación de ítems. No hubo evidencias de funcionamiento diferencial de los ítems o heterogeneidad de la población. El análisis de un modelo bifactorial mostró que los valores de los rasgos latentes son preferibles a la suma puntuaciones. La FFMQ-SF-BR es adecuada para medir el mindfulness en la población brasileña, reduciendo el tiempo de recolección de datos sin pérdida de contenido. (AU)

Análise da tendência da mortalidade por HIV/AIDS segundo características sociodemográficas no Brasil, 2000 a 2018 / Analysis of the trend of mortality from HIV/AIDS according to sociodemographic characteristics in Brazil, 2000 to 2018

Resumo A investigação analisou a tendência da mortalidade por HIV/Aids segundo características sociodemográficas nos estados brasileiros entre 2000 e 2018. Estudo ecológico de série temporal das taxas padronizadas de mortalidade por Aids geral, por sexo, faixa etária, estado civil e raça/cor. Foi utilizado o modelo linear generalizado de Prais-Winsten. Os resultados do estudo evidenciaram que os estados com as maiores taxas foram Rio Grande do Sul, Rio de Janeiro, São Paulo e Santa Catarina. A tendência foi crescente nas regiões Norte e Nordeste. Os homens tiveram taxas mais elevadas quando comparados às mulheres e à população geral. Quanto às faixas etárias, as mais avançadas mostraram tendência a crescimento. A análise de acordo com o estado civil evidenciou taxas mais elevadas entre os não casados e tendência a crescimento concentrada nesta população. De acordo com raça/cor, identificou-se que os negros apresentaram maiores taxas, exceto no Paraná, e a tendência foi majoritariamente crescente. A mortalidade por HIV/Aids apresenta tendências distintas segundo as características sociodemográficas, verificando-se necessidade de ações de prevenção e cuidado aos homens, adultos, idosos, não casados e negros em vista de mudança no perfil da mortalidade.

Abstract This investigation analyzed the trend of HIV/AIDS mortality by sociodemographic characteristics in the Brazilian states from 2000 to 2018. This is an ecological study of time-series of standardized rates of mortality from AIDS overall, by gender, age group, marital status, and ethnicity/skin color, employing the Prais-Winsten generalized linear model. The results showed that the states with the highest rates were Rio Grande do Sul, Rio de Janeiro, São Paulo, and Santa Catarina. The trend was increasing in the North and Northeast. Men had higher rates than women and the general population. The most advanced age groups showed a growing trend. The analysis by marital status showed higher and growing rates among the unmarried. Blacks had higher rates, except for Paraná, with a mainly increasing trend. Mortality due to HIV/AIDS had different trends by sociodemographic characteristics, with a need for preventive and care actions for men, adults, older adults, unmarried, and black people due to the change in the mortality profile.

Abandono do tratamento da tuberculose no Brasil, 2012-2018: tendência e distribuição espaço-temporal / Abandonment of tuberculosis treatment in Brazil, 2012-2018: trend and spatiotemporal distribution

Resumo Estudo ecológico que analisa a tendência e a distribuição espaço-temporal dos casos novos de tuberculose (TB) que abandonaram o tratamento no Brasil, notificados no Sistema de Informação de Agravos de Notificação no período de 2012 a 2018. Para o estudo da tendência, utilizou-se o modelo de regressão linear generalizada de Prais-Winsten, e para a análise espacial os índices global e local de Moran. A média e a mediana da proporção de abandono do tratamento da TB no Brasil foram de 10,4%. As maiores médias do indicador se concentraram nas regiões Sudeste (10,78 ± 1,38), Sul (10,70 ± 2,94) e Norte (10,35 ± 1,13), e nos estados de Rondônia (14,35 ± 2,34), Rio Grande do Sul (13,60 ± 4,23) e Rio de Janeiro (12,64 ± 1,73). Apenas Acre e Piauí apresentaram esse indicador abaixo de 5%. No Brasil, houve tendência de estabilidade na proporção do abandono do tratamento da TB, decréscimo nas regiões Norte, Nordeste e Sul, e somente no Distrito Federal houve crescimento. Verificou-se distribuição heterogênea e não aleatória, com cinco capitais compondo o cluster de alto-risco. Concluímos que a proporção de abandono do tratamento da TB no Brasil encontra-se acima do aceitável e que a identificação de áreas de alto risco pode contribuir para a elaboração e fortalecimento de ações de controle mais específicas.

Abstract Ecological study that analyzes the trend and the spatiotemporal distribution of new cases of tuberculosis (TB) that abandoned treatment in Brazil, notified in the Notifiable Diseases Information System, in the period from 2012 to 2018. For the study of the trend used the Prais-Winsten generalized linear regression model was used and the Moran Global and Local indices for spatial analysis. The mean and median proportion of TB treatment dropout in Brazil was 10.4%. The highest averages of the indicator were concentrated in the Southeast (10.78 ± 1.38), South (10.70 ± 2.94) and North (10.35 ± 1.13), and; in the states of Rondônia (14.35 ± 2.34), Rio Grande do Sul (13.60 ± 4.23) and Rio de Janeiro (12.64 ± 1.73), only Acre and Piauí showed this indicator below 5%. In Brazil, there was a tendency towards stability in the proportion of abandonment of TB treatment, a decrease in the North, Northeast and South regions and only in the Federal District there was growth. There was a heterogeneous and non-random distribution, with five capitals comprising the High-Risk cluster. We conclude that the proportion of TB treatment abandonment in Brazil is above what is acceptable and that the identification of high-risk areas can contribute to the elaboration and strengthening of more specific control actions.

Analysis of serum cholesterol, statins and atherosclerotic plaque in ruptured and unruptured intracranial aneurysm / Análise de colesterol sérico, estatina e placa aterosclerótica em aneurisma intracraniano roto e não roto

Introduction Intracranial aneurysm (IA) is a major healthcare concern. The use of statin to reduce serum cholesterol has shown evidence to reduce cardiovascular risk in various diseases, but the impact on IA has not been described. This study aims to determine whether statin use, and serum cholesterol levels interfere with outcomes after IA event. Methods A cohort of patients with IA was analyzed. Patients social and demographics data were collected.Modified Rankin scale (mRS) score after 6months of follow-up was the endpoint. The data regarding statins use, presence or not of atherosclerotic plaque in radiological images and serum cholesterol of 35 patients were included in our study. Linear regression models were used to determine the influence of those 6 variables in the clinical outcome. Results The prevalence of atherosclerotic plaque, high cholesterol and use of statins was 34.3%, 48.5%, and 14.2%, respectively. Statins and serum cholesterol did not impact the overall outcome,measured by mRS after 6 months (p>0.05), but did show different tendencies when separated by IA rupture status. Serum cholesterol shows na important association with rupture of aneurysm (p»0.0382). High cholesterol and use of statins show a tendency for worse outcome with ruptured aneurysm, and the opposite is true for unruptured aneurysm. The presence of atherosclerotic plaques was not related with worse outcomes. Conclusions Multiple and opposite mechanisms might be involved in the pathophysiology of IA. Ruptured aneurysms are associated with higher levels of serum cholesterol. Serum cholesterol and statins use were not correlated with worse outcomes, but further studies are important to clarify these relationships.

Introdução Aneurisma intracranial (AI) é uma grande preocupação para a saúde. Evidências apontam que o uso de estatina para reduzir o colesterol sérico diminui o risco cardiovascular em diversas doenças, mas o impacto em AI ainda não foi descrito. Este estudo almeja determinar se o uso de estatina e o nível sérico de colesterol interferem no desfecho clínico após a ocorrência de AIs. Métodos Uma coorte de pacientes com AI foi analisada. Os dados sociodemográficos dos pacientes foram coletados. Ao final de 6 meses de acompanhamento, aplicou-se a escala modificada de Rankin (mRS). Os dados sobre uso de estatina, existência de placa aterosclerótica em imagens radiológicas, e colesterol sérico de 35 pacientes foram incluídos no estudo. Modelos de regressão linear foram usados para determinar a influência dessas 6 variáveis nos desfechos clínicos. Resultados A prevalência de placa aterosclerótica, colesterol elevado, e uso de estatina foram respectivamente 34,3%, 48,5% e 14,2%. Estatina e colesterol sérico não impactaram nos desfechos medidos pela mRS em 6 meses (p > 0,05), mas mostraram diferentes tendências quando separados pelo estado de ruptura do AI. Colesterol sérico apresenta uma importante associação com ruptura de aneurisma (p » 0,0382). Colesterol elevado e uso de estatinas representam uma tendência a piores desfechos para aneurismas rompidos, e o oposto é verdade para os não rompidos. A presença de placa aterosclerótica não está relacionada com piores resultados. Conclusões Mecanismos múltiplos e opostos podem estar envolvidos na patofisio logia do AI. Aneurismas rompidos estão associados com maiores níveis de colesterol sérico. Colesterol sérico e estatinas não foram correlacionados com piores desfechos, mas mais estudos são importantes para clarificar a relação entre esses fatores

Could outcomes of intracranial aneurysms be better predict using serum creatinine and glomerular filtration rate?

Purpose: To analyze the role of serum creatinine levels as a biomarker of intracranial aneurysm outcomes. Methods: This is a prospective analysis of outcomes of patients with intracranial aneurysm. One hundred forty-seven patients with serum creatinine at admission and 6 months follow up were included. Linear and logistic regressions were used to analyze the data. Modified Rankin scale (mRS) was used to assess outcome. Results: Creatinine level was not directly related to aneurysm outcome nor aneurysm rupture (p > 0.05). However, patients with a glomerular filtration rate (GFR) lower than 72.50 mL·min­1 had an odds ratio (OR) of 3.049 (p = 0.006) for worse outcome. Similarly, aneurysm rupture had an OR of 2.957 (p = 0.014) for worse outcomes. Stepwise selection model selected 4 variables for outcomes prediction: serum creatinine, sex, hypertension and treatment. Hypertensive patients had, on average, an increase in 0.588 in mRS (p = 0.022), while treatment with microsurgery had a decrease in 0.555 (p = 0.038). Conclusions: Patients with higher GFR had better outcomes after 6 months. Patients with higher GFR had better outcomes after 6 months. Creatinine presented an indirect role in GFR values and should be included in models for outcome prediction.

Application of Linear Regression Model of Gpnmb Gene in Rat Injury Time Estimation / 法医学杂志

OBJECTIVES@#To investigate the effects of injury time, postmortem interval (PMI) and postmortem storage temperature on mRNA expression of glycoprotein non-metastatic melanoma protein B (Gpnmb), and to establish a linear regression model between Gpnmb mRNA expression and injury time, to provide aimed at providing potential indexes for injury time estimation.@*METHODS@#Test group SD rats were anesthetized and subjected to blunt contusion and randomly divided into 0 h, 4 h, 8 h, 12 h, 16 h, 20 h and 24 h groups after injury, with 18 rats in each group. After cervical dislocation, 6 rats in each group were collected and stored at 0 ℃, 16 ℃ and 26 ℃, respectively. The muscle tissue samples of quadriceps femoris injury were collected at 0 h, 12 h and 24 h postmortem at the same temperature. The grouping method and treatment method of the rats in the validation group were the same as above. The expression of Gpnmb mRNA in rat skeletal muscle was detected by RT-qPCR. The Pearson correlation coefficient was used to evaluate the correlation between Gpnmb mRNA expression and injury time, PMI, and postmortem storage temperature. SPSS 25.0 software was used to construct a linear regression model, and the validation group data was used for the back-substitution test.@*RESULTS@#The expression of Gpnmb mRNA continued to increase with the prolongation of injury time, and the expression level was highly correlated with injury time (P<0.05), but had little correlation with PMI and postmortem storage temperature (P>0.05). The linear regression equation between injury time (y) and Gpnmb mRNA relative expression (x) was y=0.611 x+4.489. The back-substitution test proved that the prediction of the model was accurate.@*CONCLUSIONS@#The expression of Gpnmb mRNA is almost not affected by the PMI and postmortem storage temperature, but is mainly related to the time of injury. Therefore, a linear regression model can be established to infer the time of injury.